Recombinant Human CXCL2/MIP-2 Protein (RPES3251)



ELISA Kit Technical Manual

Recombinant Human CXCL2/MIP-2 Protein

Background:Chemokine Ligand 2 (CXCL2) is a small secreted cytokine which belongs to the CXC chemokine family. It is secreted by monocytes and macrophages and chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. It has been known to regulate immune functions mainly by chemo-attracting neutrophils. It is produced by activated monocytes and neutrophils and expressed at sites of inflammation. It is a hematoregulatory chemokine, which suppresses hematopoietic progenitor cell proliferation. It can be induced by receptor activator of NF-kappaB ligand, the osteoclast (OC) differentiation factor, through JNK and NF-kappaB signaling pathways in OC precursor cells. CXCL2 in turn enhanced the proliferation of OC precursor cells of bone marrow-derived macrophages (BMMs) through the activation of ERK. Knockdown of CXCL2 inhibited both the proliferation of and the ERK activation in BMMs. During osteoclastogenesis CXCL2 stimulated the adhesion and the migration of BMMs. CXCL2 is a novel therapeutic target for inflammatory bone destructive diseases.
Name:Recombinant Human CXCL2/MIP-2 Protein
Assay Genie SKU:RPES3251
Synonyms:C-X-C Motif Chemokine 2; Growth-Regulated Protein Beta; Gro-Beta; Macrophage Inflammatory Protein 2-Alpha; MIP2-Alpha; CXCL2; GRO2; GROB; MIP2A; SCYB2; GRO beta; MIP2
Expression host:E.coli
Mol Mass:7.67 kDa
AP Mol Mass:10 kDa
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 400mM NaCl, pH 8.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
NCBI Summary:This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]
UniProt Code:P19875
NCBI GenInfo Identifier:127085
NCBI Gene ID:2920
NCBI Accession:P19875.1
UniProt Secondary Accession:P19875,Q6FGD6, Q9UPB8,
UniProt Related Accession:P19875
Molecular Weight:11,389 Da
NCBI Full Name:C-X-C motif chemokine 2
NCBI Synonym Full Names:C-X-C motif chemokine ligand 2
NCBI Official Symbol:CXCL2
NCBI Official Synonym Symbols:GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
NCBI Protein Information:C-X-C motif chemokine 2
UniProt Protein Name:C-X-C motif chemokine 2
UniProt Synonym Protein Names:Growth-regulated protein beta; Gro-beta
Protein Family:C-X-C motif chemokine
UniProt Gene Name:CXCL2
UniProt Entry Name:CXCL2_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
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