DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.
Product Name:
Human DFFA Recombinant Protein (RPES2441)
Product Code:
RPES2441
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
DNA Fragmentation Factor Subunit Alpha, DNA Fragmentation Factor 45 kDa Subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45, H13
Accession:
O00273
Sequence:
Met 1-Thr331
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human DNA Fragmentation Factor Subunit alpha is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
38.7 kDa
AP Mol Mass:
42 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
