EGF is one of the founding members of the EGF-family of proteins. The structural and functional features of this protein family are extremely comparable. EGF has 9 EGF-like domains and 9 LDL-receptor class B repeats. The human EGF protein is a 6045-Da molecule with 53 amino acid residues and three intramolecular disulfide bonds. EGF has first isolated from the mouse submandibular gland as a low-molecular-weight polypeptide. However, it is now present in many human tissues, including the submandibular and parotid glands. Epidermal growth factor (EGF) can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is an important protein that promotes epidermal and epithelial cell growth in vivo and in vitro. It causes cellular mitosis, differentiation, and survival. Salivary EGF, which is also regulated by dietary inorganic iodine, has an important physiological function in maintaining oro-esophageal and gastric tissue integrity. Epidermal growth factor (EGF) binds to the epidermal growth factor receptor with high affinity and activates the intrinsic protein-tyrosine kinase activity of the receptor. Tyrosine kinase activity is responsible for the activation of a signal transduction pathway that leads to several cellular biochemical changes, such as increases in intracellular calcium levels, glycolysis and protein synthesis, and increased gene expression for certain genes including the gene for EGFR - all of which lead to DNA synthesis and cell proliferation. Human EGF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.15-0.75 ng/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
6.3 kDa
AP Mol Mass:
6.3 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
