As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT). In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human EGFR/ErbB1 Recombinant Protein (RPES1169)
Product Code:
RPES1169
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
ERBB,ERBB1,HER1,mENA,NISBD2,PIG61
Accession:
NP_005219
Sequence:
Met 1-Gly 645
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 µg/ml (100 ?l/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
95 kDa
AP Mol Mass:
130-140 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.41. Normally 5 % - 8 % trehalose; mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
