Endothelial cell-selective adhesion molecule (ESAM) is a JAM family of immunoglobulin superfamily members that includes one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic. It is particularly concentrated in the endothelium and on activated platelets. ESAM at endothelial tight junctions aids neutrophil migration across the vessel wall; it's possible that it does so by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been shown to bind ESAM. ESAM has previously been shown to influence the development of primary tumour growth and endothelial permeability. ESAM appears to play a functional redundancy in physiological angiogenesis, yet it has a distinct and important function in pathological angiogenic processes such as tumour development. Human ESAM Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human ESAM Recombinant Protein (RPES0639)
Product Code:
RPES0639
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
W117m
Accession:
NP_620411.2
Sequence:
Met 1-Ala 248
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human ESAM (NP_620411.2) extracellular domain (Met 1-Ala 248) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
24.5 kDa
AP Mol Mass:
40-45 kDa
Formulation:
Lyophilized from sterile 100mM NaCl; 50mM Tris; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
