Fibroblast growth factor 7 (FGF7) is a heparin-binding growth factor that is predominately found in epithelial cells and belongs to the family of secreted proteins. The fibroblast growth factor proteins (FGF) belong to a large family of mitogenic and cell survival proteins that are involved in a wide range of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor progression and invasion. FGF7 is a potent epidermal-specific mitogen; its mitogenic activity is primarily seen in keratinocytes but not in fibroblasts or endothelial cells. It's feasible that a major paracrine effector of epithelial cell proliferation is taking place. Human FGF-7/KGF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Immobilized Human KGF at 2µg/ml(100 ?l/well) can bind Human FGF R3-Fc(Cat: PKSH033678). The ED50 of Human KGF is 0.788 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast Growth Factor 7/Keratinocyte growth factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
19.1 kDa
AP Mol Mass:
23&38 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;pH8.0;l50mM NaCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
