Fibroblast Growth Factor 12 (FGF-12) is a member of the fibroblast growth factor (FGF) family. FGF-12 is believed to be involved in the development and function of the nervous system. FGF-12 has no signal sequence at the N terminus, but it contains clusters of basic residues that have been shown to function as a nuclear localization signal. When FGF-12 was transfected into human cells, it accumulated in the nucleus but was not secreted. The precise role of FGF-12 has yet to be determined. Two alternative splicing variants of FGF-12 have been described. Human FGF2/FGF12 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Immobilized Human FGF-12 at 2ug/ml(100 ?l/well) can bind Human FGFR3-Fc(Cat: PKSH033678). The ED50 of Human FGF-12 is 1.27 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast Growth Factor 12 is produced by our E.coli expression system and the target gene encoding Met1-Thr181 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.5 kDa
AP Mol Mass:
19 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 5mM EDTA, pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
