Fibroblast growth factor 19 (FGF19) is a secreted protein which belongs to the FGFs family. FGF19 is expressed in fetal brain, cartilage, retina, and adult gall bladder. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity. FGFRs can be important for regulation of glucose and lipid homeostasis. FGF19 has important roles as a hormone produced in the ileum in response to bile acid absorption. It has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. FGF19 can be considered as a regulator of energy expenditure.
Product Name:
Human FGF9/FGF9 Recombinant Protein (RPES3911)
Product Code:
RPES3911
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Fibroblast growth factor 19, FGF-19, FGF19
Accession:
O95750
Sequence:
Phe27-Lys216
Fusion tag:
N-6His
Activity:
Immobilized Human FGF-19-His at 2µg/ml(100 ?l/well) can bind Human FGFR3-Fc(Cat: PKSH033678). The ED50 of Human FGF-19-His is 3.1135 ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 19 is produced by our E.coli expression system and the target gene encoding Phe27-Lys216 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
23.5 kDa
AP Mol Mass:
24 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB?150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
