Ferritin is a large; iron-storage heteropolymeric protein;which is expressed in most kinds of cells and co-assemble in different proportion in a tissue-specific manner. Ferritin has oligomer of 24 subunits and two types of subunits including light chain(FTL) and heavy chain. Ferritin can remove Fe (?) from solution in the presence of oxygen and is very important for iron homeostasis. Iron is absorbed in the ferrous form and deposited as ferric hydroxides after oxidation. Iron is first oxidized to the ferric state for storage as ferric oxyhdroxide whithin the protein shell of ferritin. Thus; ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.
Product Name:
Human FTL Recombinant Protein (RPES2029)
Product Code:
RPES2029
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ferritin L subunit,Ferritin light chain, FTL
Accession:
P02792
Sequence:
Met 1-Asp175
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ferritin light chain is produced by our E.coli expression system and the target gene encoding Met1-Asp175 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
24.5 kDa
AP Mol Mass:
20-25 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris;250mM NaCl;1mM EDTA;pH9.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
