Golgi membrane protein 1; also known as Golgi membrane protein GP73; Golgi phosphoprotein 2 and GOLM1; is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon; prostate; trachea and stomach. It is expressed at lower level in testis; muscle; lymphoid tissues; white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV; HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease; autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH); GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Product Name:
Human GOLPH2/GOLM1 Recombinant Protein (RPES1398)
Product Code:
RPES1398
Size:
50 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Golgi Membrane Protein 1, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, GOLM1, C9orf155, GOLPH2
Accession:
Q8NBJ4-1
Sequence:
Val 40-Leu 401
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human GOLM1 isoform 1 (Q8NBJ4-1) (Val 40-Leu 401) was fused with a polyhistidine tag at the C-terminus and a Met at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
42.7 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
