Interleukin 1 receptor type II, also known as CD121b (Cluster of Differentiation 121b), is a cytokine receptor belonging to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA); and acts as a decoy receptor that inhibits the activity of its ligands. IL-1 is a pleiotropic cytokine that controls inflammation development and maintenance. It binds to cell plasma membrane receptors and regulates the inflammatory responses. The two types of IL1 receptors that can bind IL1 specifically have been isolated, dubbed as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is said to antagonize the action of interleukin 1 by upregulating and releasing this cytokine. Human IL1R2/CD121b Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IL1R2/CD121b Recombinant Protein (RPES1934)
Product Code:
RPES1934
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD121b,CDw121b,IL-1R-2,IL-1RT-2,IL-1RT2,IL1R2c,IL1RB,Interleukin-1 receptor type 2, IL-1R-2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, IL-1R-beta, Interleukin-1 receptor type I
Accession:
NP_004624.1
Sequence:
Met 1-Glu 343
Activity:
Immobilized human IL1R2 at 10 µg/ml (100 ?l/well) can bind biotinylated human IL1B-His; The EC50 of biotinylated human IL1B-His is 0.14-0.34 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL1R2 (NP_004624.1) (Met 1-Glu 343) was expressed with five amino acids (DDDDK) at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
38.4 kDa
AP Mol Mass:
49 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
