Following the removal of the precursor signal peptide, proinsulin is cleaved into three fragments: the B chain and A chain peptides, which are linked via two disulfide bonds to form insulin, and C-peptide. Insulin binds to the insulin receptor (INSR) and stimulates glucose uptake. Human Insulin Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Insulin Recombinant Protein (RPES1561)
Product Code:
RPES1561
Size:
100 µg
Species:
Human
Expressed Host:
Yeast
Synonyms:
IDDM,IDDM1,IDDM2,ILPR,INS,IRDN,MODY10
Accession:
P01308
Sequence:
Phe1-Lys29&Gly1-Asn21
Fusion tag:
Activity:
Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 µg/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
6 kDa
AP Mol Mass:
6 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
