Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates a wide range of T and B cell responses, including cell proliferation, survival, and gene expression. IL-4 is made by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. IL-4 has a significant influence on the switching of immunoglobulins to the IgG1 and IgE isotypes. Excessive Th2 cell IL-4 activity has been linked to increased IgE production and an allergic response. Human Interleukin-4/IL-4 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-4/IL-4 Recombinant Protein (RPES3551)
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
14.97 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
