Interleukin 7 (IL-7) is a powerful lymphoid cell growth factor that stimulates the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Both human and mouse IL7 have 65% sequence identity in the mature section, and they have cross-species activity. IL-7 activates a variety of pathways via the IL-7 receptor (IL7R) that is known as the JaK/STAT and PI3K/AKT pathways. These pathways regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also involved in cytoplasmic IL2-R gamma signaling. Human Interleukin-7/IL-7 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-7/IL-7 Recombinant Protein (RPES1338)
Product Code:
RPES1338
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Interleukin-7, IL-7, IL7
Accession:
P13232
Sequence:
Asp26-His177
Activity:
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.5 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mMPB; 300mMNaCl; pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
