Lymphocyte cytosolic protein 2?LCP2?contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
Product Name:
Human LCP2 Recombinant Protein (RPES3824)
Product Code:
RPES3824
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Lymphocyte cytosolic protein 2,SH2 domain-containing leukocyte protein of 76 kDa,SLP-76 tyrosine phosphoprotein,SLP76,LCP2
Accession:
Q13094
Sequence:
Met 1-Pro533
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Lymphocyte cytosolic protein 2/SH2 Domain-containing Leukocyte Protein of 76 kDa is produced by our E.coli expression system and the target gene encoding Met1-Pro533 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
62.6 kDa
AP Mol Mass:
70 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,20% glycerol?pH 8.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
