Recombinant Human MANF/ARMET Protein (His Tag) (RPES4207)

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SKU:
RPES4207
€129

Description

ELISA Kit Technical Manual

Recombinant Human MANF/ARMET Protein (His Tag)

Background:Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. MANF enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF and a homologous protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. MANF expression was widespread in the nervous system and non-neuronal tissues.
Name:Recombinant Human MANF/ARMET Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4207
Synonyms:Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; ARMET; ARP
Species:Human
Expression host:Human Cells
Sequence:Leu25-Leu182
Accession:P55145
Mol Mass:19.2 kDa
AP Mol Mass:17 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleus

Disease: Pancreatic Cancer

NCBI Summary:The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
UniProt Code:P55145
NCBI GenInfo Identifier:332278201
NCBI Gene ID:7873
NCBI Accession:P55145.3
UniProt Secondary Accession:P55145,Q14CX4, Q86U67, Q96IS4,
UniProt Related Accession:P55145
Molecular Weight:20,700 Da
NCBI Full Name:Mesencephalic astrocyte-derived neurotrophic factor
NCBI Synonym Full Names:mesencephalic astrocyte derived neurotrophic factor
NCBI Official Symbol:MANF  
NCBI Official Synonym Symbols:ARP; ARMET  
NCBI Protein Information:mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name:Mesencephalic astrocyte-derived neurotrophic factor
UniProt Synonym Protein Names:Arginine-rich protein; Protein ARMET
Protein Family:MANF/CDNF-like protein
UniProt Gene Name:MANF  
UniProt Entry Name:MANF_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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