Description
Recombinant Human Mesothelin/MSLN Protein (aa 296-598, Fc Tag)
Background: | Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura, pericardium, and peritoneum) and epithelial cells of the trachea, tonsils, fallopian tube, and kidneys. Mesothelin plays an important role in cell survival, proliferation, migration, invasion, tumor progression, and resistance to chemotherapy. The overexpression of mesothelin can activate NF-?B and signal transducer and activator of transcription 3 (Stat3), inhibit apoptotic signaling and TNF-?-induced apoptosis, and accelerate the G1S transition. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy. |
Name: | Recombinant Human Mesothelin/MSLN Protein (aa 296-598, Fc Tag) |
Size: | 10µg |
Assay Genie SKU: | RPES4466 |
Synonyms: | Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein;CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen |
Species: | Human |
Expression host: | Human Cells |
Sequence: | Glu296-Ser598 |
Accession: | Q13421 |
Mol Mass: | 61.0 kDa |
AP Mol Mass: | 75-80 kDa |
Tag: | C-Fc |
Purity: | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months. |
Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation: | Lyophilized from a 0.2 um filtered solution of PBS, pH7.4. |
Reconstitution: | Please refer to the printed manual for detailed information. |
UniProt Protein Function: | MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Cell adhesion Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane Molecular Function:protein binding Biological Process: pancreas development; cell adhesion |
NCBI Summary: | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
UniProt Code: | Q13421 |
NCBI GenInfo Identifier: | 116242654 |
NCBI Gene ID: | 10232 |
NCBI Accession: | Q13421.2 |
UniProt Secondary Accession: | Q13421,Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2 Q9UCB2, Q9UK57, D3DU65, |
UniProt Related Accession: | Q13421 |
Molecular Weight: | 67,940 Da |
NCBI Full Name: | Mesothelin |
NCBI Synonym Full Names: | mesothelin |
NCBI Official Symbol: | MSLN |
NCBI Official Synonym Symbols: | MPF; SMRP |
NCBI Protein Information: | mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor |
UniProt Protein Name: | Mesothelin |
UniProt Synonym Protein Names: | CAK1 antigen; Pre-pro-megakaryocyte-potentiating factorCleaved into the following 2 chains:Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form |
Protein Family: | Mesothelin |
UniProt Gene Name: | MSLN |
UniProt Entry Name: | MSLN_HUMAN |
Additional Information
Product type: |
Recombinant Protein |
Host species: |
Human Cells |
Reactivity: |
Human |