Dual specificity mitogen-activated protein kinase kinase 6; also known as MAP kinase kinase 6; MAPKK 6; MAPK / ERK kinase 6; SAPKK3; MAP2K6 and MKK6; is a protein which belongs to the?protein kinase superfamily; STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors; cytokines; ultraviolet light; and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle; heart; and in lesser extent in liver or pancreas.
Product Name:
Human MKK6 Recombinant Protein (RPES1931)
Product Code:
RPES1931
Size:
50 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
MAPKK6,MEK6,MKK6,PRKMK6,SAPKK-3,SAPKK3,MAP2K6
Accession:
P52564-1
Sequence:
Met 1-Asp 334
Fusion tag:
N-His & GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human MKK6 isoform 1 (P52564-1) (Met 1-Asp 334) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Purity:
> 80 % as determined by reducing SDS-PAGE.
Mol Mass:
65.3 kDa
AP Mol Mass:
60 kDa
Formulation:
Supplied as sterile 20mM Tris; 500mM NaCl; pH 8.0
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
