Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions; such as morphogenesis; differentiation; angiogenesis and tissue remodeling; as well as pathological processes including inflammation; arthritis; cardiovascular diseases; pulmonary diseases and tumor invasion. MMP9 is a 92-kDa gelatinase B/type IV collagenase that is released by neutrophils, macrophages, and a variety of transformed cells; it's the most complex family member in terms of domain structure and control of its activity. It is an essential component of tissue remodelling in both normal and inflammatory responses. MMP-9 is an important secreted product of macrophages and a component of neutrophil cytoplasmic granules; it is especially significant in the development of inflammatory, infectious, and neoplastic diseases in many organs, including the lung. Following stimulation by inflammatory cytokines or delivery of bi-directional activation signals to integrin-mediated cell-cell or cell-extracellular matrix (ECM) interactions, this enzyme is produced by lymphocytes and stromal cells. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors; excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a result, the study of gene transcription and tissue-specific expression of MMP-9 in healthy and diseased states is being pursued to provide the groundwork for new therapeutic targets. In addition; the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention. Human MMP-9 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human MMP-9 Recombinant Protein (RPES4345)
Product Code:
RPES4345
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Matrix metalloproteinase-9,92 kDa gelatinase,92 kDa type IV collagenase,Gelatinase B,MMP9,CLG4B,GELB,MANDP2
Accession:
NP_004985.2
Sequence:
Met 1-Asp 707
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate; Mca-PLGL-Dpa-AR-NH2; (AnaSpec; Cat#27076). The specific activity is >600 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human MMP9 zymogen (NP_004985.2) (Met 1-Asp 707) was expressed.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
