Description
Recombinant Human/Mouse/Rat HMGB1 Box1 Protein
The Recombinant Human/Mouse/Rat HMGB1 Box1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Mouse/Rat HMGB1 Box1 function and its implications in health and disease.
This product (SKU: RPCB0029) is produced using HEK293 cells and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 10.4 kDa with an observed molecular weight of 14 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human/Mouse/Rat HMGB1 Box1 Protein |
| SKU: | RPCB0029 |
| Size: | 10 μg , 50 μg |
| Reactivity: | Human/Mouse/Rat |
| Synonyms: | High Mobility Group Protein B1, High Mobility Group Protein 1, HMG-1, HMGB1, HMG1, BoxA |
| Tag: | No tag |
| Expression Host: | HEK293 cells |
| Calculated MW: | 10.4 kDa |
| Observed MW: | 14 kDa |
| Gene ID: | 3146 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human/Mouse/Rat HMGB1 Box1 Protein (RPCB0029), tested reactivity in E.coli and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 0.5 mM DTT, pH 7.9. Contact us for customized product form or formulation. |
| Reconstitution: | Please contact us for reconstitution instructions. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.
 | Recombinant Human/Mouse/Rat HMGB1 Box1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
