Human NCK1 Recombinant Protein (RPES2020)
- SKU:
- RPES2020
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Signal Transduction
Frequently bought together:
Description
system_update_altDatasheet
Human NCK1 Recombinant Protein
Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
| Product Name: | Human NCK1 Recombinant Protein (RPES2020) |
| Product Code: | RPES2020 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Cytoplasmic Protein NCK1, NCK adaptor Protein 1, Nck-1, SH2/SH3 Adaptor Protein NCK-Alpha, NCK1, NCK |
| Accession: | P16333 |
| Sequence: | Met 1-Ser377 |
| Fusion tag: | N-6His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus. |
| Purity: | > 80 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 45.0 kDa |
| AP Mol Mass: | 50 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 150mM NaCl, 5% Mannitol,pH 8.0. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
