Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.
Product Name:
Human Nectin-4/NECTIN4 Recombinant Protein (RPES0140)
Product Code:
RPES0140
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
PVRL4,Nectin-4,Ig superfamily receptor LNIR,Poliovirus receptor-related protein 4,PRR4,LNIR
Accession:
Q96NY8
Sequence:
Gly31-Val351
Fusion tag:
C-6His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of NIH?3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 µg/mL with 100 µL/well), approximately 90% will adhere after 1 hour incubation at 37 °C.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly31-Val351 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
35.3 kDa
AP Mol Mass:
40-47 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
