Description
Recombinant Human NF-kB p65/RELA Protein
The Recombinant Human NF-kB p65/RELA Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating NF-kB p65/RELA function and its implications in health and disease.
This product (SKU: RPCB2024) is produced using E. coli and features a N-GST tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 62 kDa with an observed molecular weight of 58 kDa under denaturing conditions, achieving ≥ 70 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human NF-kB p65/RELA Protein |
| SKU: | RPCB2024 |
| Size: | 100 μg |
| Reactivity: | Human |
| Synonyms: | NFKB3, RELA |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Calculated MW: | 62 kDa |
| Observed MW: | 58 kDa |
| Gene ID: | 5970 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human NF-kB p65/RELA Protein (RPCB2024), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | Please contact us for more information. |
| Purity: | ≥ 70 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 0.15M NaCl, 20mM GST, pH 8.0 |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in
 | Recombinant Human NF-kB p65/RELA Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
