Nucleolar protein 3 is encoded by NOL3 gene. Multiple transcript variants exist for this gene, each of which encodes a different isoform. So far, two isomeric forms of Nucleolar protein 3 have been discovered. Isoform 1 is thought to be involved in RNA splicing. Isoform 2 is an apoptosis repressor that inhibits a variety of cell death pathways. It inhibits extracellular apoptosis pathways in two ways by inhibiting both intrinsic and extrinsic apoptotic mechanisms. First, by interacting with FAS and FADD following FAS activation, it inhibits the formation of the death-inducing signaling complex (DISC) . Finally, through their interactions with CASP8 in a manner that is dependent on mitochondrial localization and phosphorylation, they act to limit soluble CASP8 available for DISC-mediated activation. It has been found to downregulate caspase 2, caspase 8, and tumor protein p53 enzyme activities. Human NOL3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human NOL3 Recombinant Protein (RPES0771)
Product Code:
RPES0771
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Nucleolar Protein 3, Apoptosis Repressor With CARD, Muscle-Enriched Cytoplasmic Protein, Myp, Nucleolar Protein of 30 kDa, Nop30
Accession:
O60936
Sequence:
Met 1-Ser208
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Nucleolar Protein 3 is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
22.6 kDa
AP Mol Mass:
29 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 25mM TrisHCl; 1mM DTT; 1mM EDTA; 2mM ?-ME; 20% Glycerol; pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
