PR domain containing 2; with ZNF domain (PRDM2); also known as zinc finger protein RIZ; is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain; which shares sequence homology with an independently described sequence motif; the PR domain. PRDM2 is a protein that has eight C2H2-type zinc fingers and a unique SET domain, as well as being highly expressed in retinoblastoma cell lines and brain tumors; it's also found in different cell lines and brains, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3; and is identified as a tumor suppressor. It is thought that the PR( SET) sequence is essential for tumor suppression activities; in human malignancies, changes in the PR domain resulted in reduced activity. Additionally, S-adenosylhomocysteine or a methyl donor deficit impairs RIZ1 and other H3 lysine 9 methylation functions. PRDM2 may also function as a DNA-binding transcription factor. It binds to the macrophage-specific TPA-responsive element (MTE) in the HMOX1 (heme oxygenase 1) gene and acts as a transcriptional activator. PRDM2 (RIZ) binds to the retinoblastoma protein (RB) and interacts with GATA3, in addition. Human PRDM2/RIZ1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human PRDM2/RIZ1 Recombinant Protein (RPES2819)
Product Code:
RPES2819
Size:
100 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
HUMHOXY1,KMT8,MTB-ZF,RIZ,RIZ1,RIZ2
Accession:
NP_036363.2
Sequence:
Met 1-Ala 200
Fusion tag:
Endotoxin:
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Protein Construction:
A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200); containing the SET domian; was expressed; with two additonal amino acids (Gly & Pro) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
