Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A); and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily; RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver; and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma; the RBP4-retinol complex interacts with transthyretin (TTR); and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes; and its deficiency affects night vision largely. Recently; RBP4 as an adipokine; is found to be expressed in adipose tissue and correlated with obesity; insulin resistance (IR) and type 2 diabetes (T2DM).
Product Name:
Human RBP4 Recombinant Protein (RPES3926)
Product Code:
RPES3926
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
RDCCAS,Retinol-Binding Protein 4, Plasma Retinol-Binding Protein, PRBP, RBP, RBP4
Accession:
NP_006735.2
Sequence:
Met 1-Leu 201
Fusion tag:
C-His
Activity:
Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.The 50% binding concentration (BC50) is > 1.0 µM
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the pro form of human RBP4 (NP_006735.2) (Met 1-Leu 201) was expressed with a C-terminal polyhistidine tag.
Purity:
> 85 % as determined by reducing SDS-PAGE.
AP Mol Mass:
23 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.2
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
