Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Product Name:
Human SMAD3 Recombinant Protein (RPES4825)
Product Code:
RPES4825
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3
Accession:
P84022
Sequence:
Ser2-Ser425
Fusion tag:
N-6His-Flag
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Mothers Against Decapentaplegic Homolog 3 is produced by our E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
50.3 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,500mM NaCl, pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetMouse SMAD3 Recombinant Protein SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily fr
Overview Inflammation ELISA Sampler Kit The Inflammation ELISA Sampler Kit from Assay Genie allows researchers to begin their investigation into Inflammation via analysing IL-1 Beta, IL-1RA,...
Overview MAPK Family ELISA Sampler PackMAPK Family ELISA Sampler Pack from Assay Genie allows researchers to begin their investigation into MAPK Family via analysing p38 MAPK, JNK, ERK1 / MAPK3,...
Overview Cancer Associated Fibroblast Marker ELISA Sampler PackCancer Associated Fibroblast Marker ELISA Sampler Pack from Assay Genie allows researchers to begin their investigation into Cancer...
Overview Toll-like Receptor ELISA Kit PackToll-like Receptor ELISA Kit Pack from Assay Genie allows researchers to begin their investigation into Toll-like Receptor via analysing TLR3 / Toll-like...
Overview Akt Pathway ELISA Sampler PackAkt Pathway ELISA Sampler Pack from Assay Genie allows researchers to begin their investigation into Akt Pathway via analysing PDK1, PTEN, AKT2, AKT3, AKT1,...
Overview TGF-β Fibrosis Pathway ELISA Kit PackTGF-β Fibrosis Pathway ELISA Kit Pack from Assay Genie allows researchers to begin their investigation into TGF-β Fibrosis Pathway via analysing...
Overview Senescence Marker ELISA Sampler PackSenescence Marker ELISA Sampler Pack from Assay Genie allows researchers to begin their investigation into Senescence Marker via analysing IL-6, CDKN2A...
