SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Product Name:
Human SMAD4 Recombinant Protein (RPES5146)
Product Code:
RPES5146
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Mothers Against Decapentaplegic Homolog 4, MAD Homolog 4, Mothers Against DPP Homolog 4, Deletion Target in Pancreatic Carcinoma 4, SMAD Family Member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Accession:
Q13485
Sequence:
Met 1-Asp552
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Mothers Against Decapentaplegic Homolog 4 is produced by our E.coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
61.5 kDa
AP Mol Mass:
62 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl?pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
