Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Product Name:
Human Thrombopoietin/TPO Recombinant Protein (RPES0736)
Product Code:
RPES0736
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Thrombopoietin,C-mpl ligand,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,Myeloproliferative leukemia virus oncogene ligand,THPO
Accession:
P40225
Sequence:
Ser22-Gly353
Fusion tag:
N,C-6His
Activity:
Measured in a cell proliferation assay using MO7E human megakaryocytic leukemic cells. The ED50 for this effect is 0.55 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Thrombopoietin is produced by our Mammalian expression system and the target gene encoding Ser22-Gly353 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
37.3 kDa
AP Mol Mass:
70-90 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
