T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the CD28 family within the Ig superfamily of proteins. TIGIT is expressed on NK cells and subsets of activated; memory and regulatory T cells; and particularly on follicular helper T cells within secondary lymphoid organs. It binds to CD155 and Nectin-2 that appear on dendritic cells (DC) and endothelium. Ligation of TIGIT on T cells down-regulates TCR-mediated activation and subsequent proliferation; while NK cell TIGIT ligation blocks NK cell cytotoxicity. Through CD155 and Nectin-2; which also interact with DNAM-1/CD226 and CD96/Tactile; TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other. In particular; TIGIT binding to CD155 can antagonize the effects of DNAM1.
Product Name:
Human TIGIT Recombinant Protein (RPES3371)
Product Code:
RPES3371
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
T-cell immunoreceptor with Ig and ITIM domains,,VSIG9, VSTM3,TIGIT,V-set and transmembrane domain-containing protein 3,V-set and immunoglobulin domain-containing protein 9
Accession:
Q495A1
Sequence:
Met22-Pro141
Fusion tag:
C-6His
Activity:
Immobilized Human PVR-Fc(Cat: PKSH033562) at 10µg/ml(100 ?l/well) can bind Human TIGIT-His. The ED50 of Human TIGIT-His is 8.2 ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human T cell Immunoreceptor with Ig and ITIM domains is produced by our Mammalian expression system and the target gene encoding Met22-Pro141 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
14.1 kDa
AP Mol Mass:
16-18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
