Thymidine kinase 1(TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser-13 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5'-phosphate with the help of ATP
Product Name:
Human TK1 Recombinant Protein (RPES0789)
Product Code:
RPES0789
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Thymidine kinase, cytosolic,TK1
Accession:
P04183
Sequence:
Met 1-Asn234
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Thymidine kinase, Cytosolic is produced by our Mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
26.5 kDa
AP Mol Mass:
28 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,1mM DTT,2mM EDTA, 10% Glycerol, pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
