Human TK1 Recombinant Protein (RPES0789)

SKU:
RPES0789
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€361

Description

ELISA Kit Technical Manual

Recombinant Human TK1 Protein (His Tag)

Background:Thymidine kinase 1(TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser3 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5'-phosphate with the help of ATP
Name:Recombinant Human TK1 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES0789
Synonyms:Thymidine kinase; cytosolic;TK1
Species:Human
Expression host:Human Cells
Sequence:Met 1-Asn234
Accession:P04183
Mol Mass:26.5 kDa
AP Mol Mass:28 kDa
Tag:C-6His
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,1mM DTT,2mM EDTA, 10% Glycerol, pH 7.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
UniProt Protein Details:

Protein type:EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function:identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation

UniProt Code:P04183
NCBI GenInfo Identifier:23503074
NCBI Gene ID:7083
NCBI Accession:P04183.2
UniProt Secondary Accession:P04183,Q969V0, Q9UMG9, B2RC58,
UniProt Related Accession:P04183
Molecular Weight:234
NCBI Full Name:Thymidine kinase, cytosolic
NCBI Synonym Full Names:thymidine kinase 1, soluble
NCBI Official Symbol:TK1
NCBI Official Synonym Symbols:TK2
NCBI Protein Information:thymidine kinase, cytosolic; thymidine kinase; thymidine kinase 1 soluble isoform
UniProt Protein Name:Thymidine kinase, cytosolic
Protein Family:Thymidine kinase
UniProt Gene Name:TK1
UniProt Entry Name:KITH_HUMAN