The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.
Immobilized Human NT-3(Cat: PKSH032803) at 2µg/ml(100 ?l/well) can bind Human TrkB-His. The ED50 of Human TrkB-His is 3.777ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human TrkB is produced by our Mammalian expression system and the target gene encoding Cys32-His430 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
45.25 kDa
AP Mol Mass:
60-105 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
