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Human UBE2D1 Recombinant Protein (RPES2834)

SKU:
RPES2834
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
€34
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Description

ELISA Kit Technical Manual

Recombinant Human UBE2D1 Protein (GST Tag)

Background:Ubiquitin-conjugating enzyme E2 D1?UBE2D1?belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Name:Recombinant Human UBE2D1 Protein (GST Tag)
Size:10µg
Assay Genie SKU:RPES2834
Synonyms:Ubiquitin-conjugating enzyme E2 D1;Stimulator of Fe transport;SFT;UBC4/5 homolog;UbcH5;Ubiquitin carrier protein D1;Ubiquitin-conjugating enzyme E2(17)KB 1;Ubiquitin-conjugating enzyme E27 kDa 1;Ubiquitin-protein ligase D1;SFT; UBC5A; UBCH5; UBCH5A
Species:Human
Expression host:E.coli
Sequence:Met 1-Met 147
Accession:P51668
Mol Mass:42.9 kDa
AP Mol Mass:25&50 kDa
Tag:N-GST
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 50mM HEPES,150mM NaCl,2mM DTT,10% Glycerin,pH7.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:UBE2D1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol; ubiquitin ligase complex

Molecular Function:protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; BMP signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; transforming growth factor beta receptor signaling pathway; mitotic cell cycle spindle assembly checkpoint; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; toll-like receptor 4 signaling pathway

NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:P51668
NCBI GenInfo Identifier:1717848
NCBI Gene ID:7321
NCBI Accession:P51668.1
UniProt Related Accession:P51668
Molecular Weight:
NCBI Full Name:Ubiquitin-conjugating enzyme E2 D1
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 D1
NCBI Official Symbol:UBE2D1  
NCBI Official Synonym Symbols:SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 D1
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 D1
UniProt Synonym Protein Names:Stimulator of Fe transport; SFT; UBC4/5 homolog; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E27 kDa 1; Ubiquitin-protein ligase D1
UniProt Gene Name:UBE2D1  
UniProt Entry Name:UB2D1_HUMAN