ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Product Name:
Human ZWINT Recombinant Protein (RPES3087)
Product Code:
RPES3087
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT
Accession:
O95229
Sequence:
Met 1-Pro277
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human ZW10 Interactor is produced by our E.coli expression system and the target gene encoding Met1-Pro277 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
33.4 kDa
AP Mol Mass:
36 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
