The Recombinant Iba1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110622) against Iba1. This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Mouse spleen and IHC analysis of Human liver tissue, Mouse liver tissue, Human brain. The target is localized to the cytoplasm.
IHC 1:2000-4000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P55008
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human liver tissue using Recombinant Iba1 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded mouse liver tissue using Recombinant Iba1 Monoclonal Antibodyusing at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human brain Recombinant Iba1 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Iba1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Mouse spleen cells. Observed-MW:17 kDa Calculated-MW:17 kDa