The Recombinant IGFBP7 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C310051) against IGFBP7. This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC-P, ICC/IF and FC applications. It has been validated by WB analysis of Jurkat, 293T, MCF7, IHC analysis of Human prostate, Human lung cancer and IF analysis of MCF-7. The target is localized to the secreted.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of MCF-7 cells labelling IGFBP7. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human lung tissue sections using IGFBP7 Antibody (RMEA0065) at a dilution of 1:150 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Human prostate tissue sections using IGFBP7 Antibody (RMEA0065) at a dilution of 1:150 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of Jurkat,293T and MCF7 cell lysates using IGFBP7 Antibody (RMEA0065) at a dilution of 1:1500. Observed-MW:29 kDa Calculated-MW:29 kDa