The Recombinant ING5 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170171) against ING5. This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, ICC/IF, FC and IP applications. It has been validated by WB analysis of HeLa, MCF7, A549, HepG2, Mouse kindey, C6, Rat brain, Rat kindey and IF analysis of HeLa.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of HeLa cells labelling ING5. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
Western blot analysis of HeLa,MCF7,A549,HepG2,Mouse kindey,C6,Rat brain and Rat kindey cell lysates using ING5 Antibody (RMEA0185) at a dilution of 1:1000. Observed-MW:32 kDa Calculated-MW:28 kDa