The Recombinant Integrin β1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110482) against Integrin β1. Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of 3T3-L1 and IHC analysis of Mouse stomach, Human hepatocellular carcinoma. The target is localized to the membrane.
IHC 1:2000-10000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P05556
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded mouse stomach using Recombinant Integrin β1 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human hepatocellular carcinoma using Recombinant Integrin β1 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Integrin β1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: 3T3-L1 cells. Observed-MW:115-125 kDa Calculated-MW:88 kDa
