The Recombinant IRF-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110928) against IRF-1. IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse, Rat and Fish samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Mouse brain. The target is localized to the nucleus, cytoplasm, MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1.
IHC 1:100-400; WB 1:500-2000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P10914
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant IRF-1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Mouse brain whole cell lysate. Observed-MW:48 kDa Calculated-MW:37 kDa
