The Recombinant IRS-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110945) against IRS-1. This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse, Rat and Pig samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of C6. The target is localized to the nucleus, cytoplasm, cytosol, plasma membrane, insulin receptor complex, caveola, intracellular membrane-bounded organelle.
IHC 1:400-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P35568
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant IRS-1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: C6 whole cell lysate. Observed-MW:180 kDa Calculated-MW:131 kDa
