The Recombinant Keratin 4 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311202) against Keratin 4. There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of C6, HeLa and IF analysis of A431. The target is localized to the cytoplasm.
WB 1:1000-5000; IHC-P 1:100-200; ICC/IF 1:50-100; IP 1:10-100; FCM 1:100-500
UniProt:
P19013
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of A431 cells using Keratin 4 Antibody(RMEA1202). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
Western blot analysis of C6 and HeLa cell lysates using Keratin 4 Antibody (RMEA1202) at a dilution of 1:1000. Observed-MW:57 kDa Calculated-MW:57 kDa
