The Recombinant KLF4 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110716) against KLF4. This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HCT-116 and IHC analysis of Human colon tissue, Mouse colon tissue. The target is localized to the nuclear.
Product Name:
Recombinant KLF4 Monoclonal Antibody
SKU:
AGEL6024
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
EZF, GKLF, KLF, KLF4
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human KLF4 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110716
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
KLF4
Conjugation:
Unconjugated
Observed MW:
60 kDa
Calculated MW:
55 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
O43474
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human colon tissue using Recombinant KLF4 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded mouse colon tissue using Recombinant KLF4 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant KLF4 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HCT-116 cells. Observed-MW:60 kDa Calculated-MW:55 kDa