The Recombinant Lactate Dehydrogenase Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110692) against Lactate Dehydrogenase. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Human liver tissue, Rat liver tissue. The target is localized to the cytoplasmic, membranous.
Cell proliferation-inducing gene 19 protein, GSD, GSD11, HEL-S-133P, L lactate dehydrogenase A chain, L-Lactate Dehydrogenase A Chain, l7R, l7R2, Lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH-A, LDH-M, LDH1, LDH5, LDHA, LDHM, PIG, PIG 19, PIG19, PIG19 Proliferation-inducing gene, PIG19 Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Lactate Dehydrogenase protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110692
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Lactate Dehydrogenase
Conjugation:
Unconjugated
Observed MW:
37 kDa
Calculated MW:
37 kDa
Recommended Dilution:
IHC 1:1000-3000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P00338, P07195, P07864
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human liver tissue using Recombinant Lactate Dehydrogenase Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat liver tissue using Recombinant Lactate Dehydrogenase Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Lactate Dehydrogenase Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cells. Observed-MW:37 kDa Calculated-MW:37 kDa