The Recombinant LIMK1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331663) against LIMK1. LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of U-87 MG,?K562, SK-N-BE(2), Ramos. The target is localized to the cytoplasm, nucleus.
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C331663
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
LIMK1
Conjugation:
Unconjugated
Observed MW:
70 kDa
Calculated MW:
73 kDa
Recommended Dilution:
WB 1:2000
UniProt:
P53667
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with LIMK1 Monoclonal Antibody at dilution of 1:2000. Lane 1: U-87 MG, Lane 2: K562, Lane 3: SK-N- BE(2), Lane 4: Ramos Observed-MW:70 kDa Calculated-MW:73 kDa
