Angiopoietin-likeProtein 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins. Mature mouse ANGPTL3 contains an N-terminalcoiled coil domain and a C-terminalfibrinogen-likedomain. Within the Nterminalfragment, mouse ANGPTL3 shares 83% and 92% aa sequence identity with human and rat ANGPTL3, respectively. ANGPTL3 is expressed in the liver from early in development through adulthood. ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymesresponsible for hydrolyzing circulating triglycerides and HDL phospholipids. This activity requires a putative heparin-bindingmotif which is N-terminalto thecoiled coil domain. Proteolytic removal of the fibrinogen-likedomain from the N-terminalfragment serves to activate ANGPTL3 and increase its ability toinhibit LPL in vitro and function in vivo. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion oruptake. ANGPTL3 expression in vivo is up-regulatedby LXR agonists anddown-regulatedby insulin, leptin, and agonists of TRβ or PPARβ. ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells.
Product Name:
Mouse ANGPTL3 Recombinant Protein (RPES2198)
Product Code:
RPES2198
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Angiopoietin-related Protein 3, Angiopoietin-like protein 3, Angptl3
Accession:
Q9R182
Sequence:
Ser17-Thr206
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse ANGPTL3 is produced by our Mammalian expression system and the target gene encoding Ser17-Thr206 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
22.7 kDa
AP Mol Mass:
25-30 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
