Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Product Name:
Mouse E-Cadherin/CDH1 Recombinant Protein (RPES0332)
Product Code:
RPES0332
Size:
100 µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AA960649,ARC-1,E-cad,Ecad,L-CAM,Um,UVO
Accession:
P09803
Sequence:
Met 1-Val 709
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Mouse Cadherin-1 coated plates (0.8 µg/mL with 100 ?L/well), approximately >50% will adhere after 1 hour at 37?.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
78 kDa
AP Mol Mass:
80-100 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
