Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
Product Name:
Mouse Glypican/GPC1 Recombinant Protein (RPES0884)
Product Code:
RPES0884
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Glypican-1,Gpc1
Accession:
Q9QZF2
Sequence:
Asp24-Ser529
Fusion tag:
C-6His
Activity:
Immobilized Human FGFb(Cat: PKSH032437) at 2µg/ml(100 ?l/well) can bind Mouse GPC1-His. The ED50 of Mouse GPC1-His is 786 ng/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Glypican-1 is produced by our Mammalian expression system and the target gene encoding Asp24-Ser529 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
56.7 kDa
AP Mol Mass:
56kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
