Mouse Notch1 is a 300 kDa type I transmembrane glycoprotein and it functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Mouse Notch1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGFlike repeats and three Lin12/notch repeats, a 21 aa transmembrane segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Notch1 may play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation and may be involved in mesoderm development, somite formation and neurogenesis.
Product Name:
Mouse Motch A/NOTCH1 Recombinant Protein (RPES1014)
Product Code:
RPES1014
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Neurogenic locus notch homolog protein 1, Notch 1, Motch A, Mt14,lin-12,Mis6,N1,Tan1
Accession:
Q01705
Sequence:
Ala18-Gln526
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Notch 1 is produced by our Mammalian expression system and the target gene encoding Ala18-Gln526 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
54.4 kDa
AP Mol Mass:
70 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
