S100A4 is a member of the S100 family of proteins. The S100 family is further classified as a member of the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium-dependent and calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two ?-helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein. Mouse S100A4 is 101 amino acids (aa) in length. It contains two EF hand domains, one between aa 12-47, and a second between aa 50-85. S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation.
Product Name:
Mouse S100A4 Recombinant Protein (RPES0759)
Product Code:
RPES0759
Size:
10µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
Protein S100-A4, Metastasin, Metastatic cell protein, PEL98, Placental calcium-binding protein, Protein 18A2, Protein Mts1, S100 calcium-binding protein A4, S100a4, Capl, Mts1
Accession:
P07091
Sequence:
Met1-Lys101
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse S100-A4 is produced by our E.coli expression system and the target gene encoding Met1-Lys101 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
12.5 kDa
AP Mol Mass:
13 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
