Stromal derived factors (SDFs) are a loosely defined group of molecules that are generated by stromal cells. Two of the stromal derived factors, SDF-1 and SDF-4 belong to the chemokine family. Other SDFs, such as SDF-2 and SDF-5 are not well defined and their biological functions are less known. SDF-2 is first isolated from the mouse stromal cell line ST2 as a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92 %, and the aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. SDF-1 and its receptor are strongly indicated in the progression of various cancers including breast cancer. SDF-2, SDF2-L1, SDF-4, and SDF-5 are ubiquitously expressed in various cancer cell lines and SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues. These SDFs have prognostic value and warrant further investigation in their biological functions and clinical value.
Product Name:
Mouse SDF2 Recombinant Protein (RPES3501)
Product Code:
RPES3501
Size:
20µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
AI853825
Accession:
Q9DCT5
Sequence:
Met 1-Leu 211
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse SDF2 (Q9DCT5) (Met 1-Leu 211) was expressed?with a C-terminal polyhistidine tag.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
22.8 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
